Journal article
Quantitative proteomic characterization of cellular pathways associated with altered insulin sensitivity in skeletal muscle following high-fat diet feeding and exercise training
M Kleinert, BL Parker, TE Jensen, SH Raun, P Pham, X Han, DE James, EA Richter, L Sylow
Scientific Reports | NATURE PORTFOLIO | Published : 2018
Abstract
Regular exercise elicits advantageous metabolic adaptations in skeletal muscle, such as improved insulin sensitivity. However, the underpinning molecular mechanisms and the effect of diet on muscle exercise training benefits are unclear. We therefore characterized the skeletal muscle proteome following exercise training (ET) in mice fed chow or high-fat diet (HFD). ET increased exercise performance, lowered body-weight, decreased fat mass and improved muscle insulin action in chow-and HFD-fed mice. At the molecular level, ET regulated 170 muscle proteins in chow-fed mice, but only 29 proteins in HFD-fed mice. HFD per se altered 56 proteins, most of which were regulated in a similar direction..
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Awarded by Novo Nordisk Fonden
Funding Acknowledgements
We acknowledge the skilled technical assistance of Betina Bolmgren, Nicoline Resen Andersen (Molecular Physiology Group, Department of Nutrition, Exercise and Sports, University of Copenhagen, Denmark) and Emilija Malogajski (Institute for Diabetes and Obesity, Helmholtz Diabetes Center at Helmholtz Zentrum Munchen). We also acknowledge Sara Brandt for her help with editing the manuscript (Institute for Diabetes and Obesity, Helmholtz Diabetes Center at Helmholtz Zentrum Munchen). L6-GLUT4myc muscle cells were a kind gift from Amira Klip (Cell Biology Program, Research Institute, The Hospital for Sick Children, Toronto, Canada). The study was supported by several grants. Danish Research Council (DFF - 4004-00233 to L.S.; DFF - 5053-00155 to M. K.; and DFF-6108-00203A to E.A.R.). The Novo Nordisk Foundation (grant number: 27274 to E.A.R.). Novo Nordisk Foundation Excellence project grant to T.E.J. (# 15182) and L.S. (# 32082). B.L.P. is an NHMRC Early Career Fellow. D.E.J. is an NHMRC Senior Principal Research Fellow and this work was supported by an NHMRC Project Grant (APP1122376). The contents of the published material are solely the responsibility of the individual authors and the Administering Institution and do not reflect the views of the NHMRC.